miR-4770 promotes colorectal cancer progression by targeting PRKAA2 via m6A modification

Figure 2 The expression of miR-4770 in colorectal cancer and its effect on cell viability, migration and invasion. A: The expression of miR-4770 in colorectal cancer (CRC) tissues and adjacent normal tissues was detected by qRT-PCR; B: The expression of miR-4770 in FHC and HCT116 cells was detected by qRT-PCR; C: The expression of miR-4770 in the NC inhibitor, miR inhibitor, NC mimic, and miR mimic groups was detected by qRT-PCR; D: CCK-8 method was used to detect the proliferation activity of the above four groups of cells at 24 hours, 48 hours, and 72 hours; E and F: Transwell assay was used to evaluate the migration (E) and invasion (F) abilities of the above four groups of cells. Scale: 100 μm. The data were expressed as mean ± SD. ^aP < 0.01 vs normal group; ^bP < 0.01 vs FHC group; ^cP < 0.05 and ^dP < 0.01 vs NC inhibitor group; ^eP < 0.05 and ^fP < 0.001 vs NC mimic group. Clinical tissue analysis included 10 paired CRC and adjacent normal tissues, and in vitro experiments were performed with three independent biological replicates. CRC: Colorectal cancer.