Correlation between KAT6A and PD-L1 expression and role of KAT6A in colorectal cancer

Figure 5 Pharmacological inhibition of KAT6A recapitulates genetic knockdown phenotypes. A: Dose-dependent suppression of H3K23pr and PD-L1 by WM-3835. Western blot (left) and densitometric quantification (right) showed H3K23pr and PD-L1 protein levels in SW480/SW620 cells treated with WM-3835 (0, 5, or 10 μM) for 48 hours; B: PD-L1 transcriptional downregulation as revealed by quantitative PCR analysis of PD-L1 mRNA levels; C: Enhanced T cell-mediated apoptosis as shown by flow cytometry quantification of apoptotic colorectal cancer cells co-cultured with activated T cells; D: Activation of T cell cytotoxic markers demonstrated by Western blot analysis of GZMB and perforin in T cells. Data represent the mean ± SEM (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs DMSO (one-way ANOVA with Dunnett’s test). siKAT6A data from Figures 1 and 2 are shown as positive controls.