Deoxycholic acid induces reactive oxygen species accumulation and promotes colorectal cancer cell apoptosis through the CaMKII-Ca2+ pathway

Figure 4 Effect of intracellular calcium chelator BAPTA-AM on intracellular and mitochondrial Ca²⁺ and reactive oxygen species. A: Flow cytometry results of intracellular calcium content in SW480 cells; B: Flow cytometry results of intracellular calcium content in mitochondria of SW480 cells; C and D: Flow cytometry results and statistics of the calcium ion content in the mitochondria of SW480 cells; E and F: Flow cytometry results and statistical graphs of reactive oxygen species (ROS) content in mitochondria of SW480 cells. The effect of deoxycholic acid on the mitochondrial Ca²⁺ and ROS in SW480 cells after chelating intracellular calcium ions at 0, 1, and 2.5 μM in three replicates of BAPTA-AM treatment, respectively. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. PE: Phycoerythrin; FITC: Fluorescein isothiocyanate; DCA: Deoxycholic acid.