GEN1 regulates cell proliferation, migration, apoptosis and ferroptosis in gastric cancer

Figure 7 GEN1 knockdown sensitizes gastric cancer cells to ferroptosis and cisplatin. A: Ferrostatin-1 rescues cell viability in GEN1-knockdown AGS cells. Data are mean ± SD (n = 3); B: Dose-response curves of cisplatin in negative control with scrambled shRNA and shGEN1 AGS cells. Half-maximal inhibitory concentration values: ShCtrl = 5.9 μM, shGEN1 = 2.8 μM (P < 0.001, unpaired t-test). ^bP < 0.01. ^cP < 0.001. ¹P vs negative control with scrambled shRNA. ²P vs shGEN1 (two-way analysis of variance). shCtrl: Negative control with scrambled shRNA; sh: Short hairpin; IC₅₀: Half-maximal inhibitory concentration.