GEN1 regulates cell proliferation, migration, apoptosis and ferroptosis in gastric cancer

Figure 5 Enrichment analysis of GEN1-related genes. A: The Kyoto Encyclopedia of Genes and Genomes enrichment analysis was applied with 264 genes associated with GEN1 (r > 0.4, P < 0.001); B: Visualization of the genes of the cell cycle pathway associated with GEN1 through cytoscape. Red represented a positive correlation, and the darker the color, the stronger the correlation; C: Western blotting detected CCNA2, CCNB1, CCNB2, cyclin-dependent kinase (CDK) 1, CDK2 and polo-like kinase 1 protein levels in AGS cells transfected with negative control with scrambled shRNA cells or shGEN1 (n = 3). sh: Short hairpin; CDK: Cyclin-dependent kinase; PLK1: Polo-like kinase 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; ATP: Adenosine triphosphate.