GEN1 regulates cell proliferation, migration, apoptosis and ferroptosis in gastric cancer

Figure 2 The interference efficiency of shGEN1 on GEN1 in AGS cells. A: Observation of the state of negative control with scrambled shRNA and shGEN1 infected cells after 72 hours under a fluorescence microscope; B: Real-time quantitative polymerase chain reaction detected the expression levels of GEN1 mRNA after lentiviral infection (middle) (n = 3); C: Western blotting detected GEN1 protein levels after lentiviral infection (right) (n = 3). ^bP < 0.01. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GFP: Green fluorescent protein; shCtrl: Negative control with scrambled shRNA; sh: Short hairpin.