Mesenchymal stem cell-derived lncRNAs NKILA contributes to stemness and chemoresistance by fatty acid oxidation in gastric cancer via miR-485-5p/STAT3

Figure 6 NKILA induces fatty acid oxidation of gastric cancer cells by targeting miR-485-5p. The MKN45 and AGS cells were transfected NKILA and co-treated with miR-485-5p mimic. A: The CPT1 activity was detected in the cells; B: The β-oxidation was analyzed in the cells; C: The relative ATP levels was determined in the cells; D: The mRNA expression of SOX2, Oct4, LIN28, and CD133 was measured by qPCR; E: The stemness was analyzed using sphere formation assay; F and G: The chemoresistance of oxaliplatin or 5-Fu was detected by CCK-8. ^aP < 0.01.