Mesenchymal stem cell-derived lncRNAs NKILA contributes to stemness and chemoresistance by fatty acid oxidation in gastric cancer via miR-485-5p/STAT3

Figure 5 NKILA enhances STAT3 expression by targeting miR-485-5p in gastric cancer cells. A: The interaction site of miR-485-5p between NKILA and STAT3; B and C: The MKN45 and AGS cells were transfected miR-485-5p mimic. The expression of miR-485-5p was measured by qPCR (B). The luciferase activity of NKILA was analyzed by Luciferase reporter gene assay (C); D: The direct interaction of miR-485-5p and NKILA was analyzed by RNA pull down; E: The luciferase activity of STAT3 3'UTR was analyzed by Luciferase reporter gene assay; F: The expression of STAT3 was measured by qPCR in MKN45 and AGS cells were transfected miR-485-5p mimic; G: The expression of STAT3 was measured by qPCR in MKN45 and AGS cells were transfected miR-485-5p mimic and NKILA overexpression plasmid. ^aP < 0.01.