Mesenchymal stem cell-derived lncRNAs NKILA contributes to stemness and chemoresistance by fatty acid oxidation in gastric cancer via miR-485-5p/STAT3

Figure 2 Mesenchymal stromal cells-derived long non-coding RNAs NKILA promotes stemness and chemoresistance of gastric cancer cells. A: The expression of NKILA was detected by qPCR in clinical gastric cancer tissue and non-tumor tissues; B: The expression of NKILA was determined by qPCR inMKN45 and AGS cells co-cultured with mesenchymal stromal cells; C-F: The MKN45 and AGS cells were transfected with NKILA overexpressing plasmid; The mRNA expression of SOX2, Oct4, LIN28, and CD133 was measured by qPCR (C and D); The CD44+ and CD133+ cells were identified by flow cytometry analysis (E); The stemness was analyzed using sphere formation assay (F); G and H: The MKN45 and AGS cells were transfected with NKILA overexpressing plasmid and treated with oxaliplatin or 5-Fu; The cell apoptosis was determined by flow cytometry analysis (G); The cell proliferation was examined by colony formation assay (H). ^aP < 0.01; MSC: Mesenchymal stromal cell.