Mesenchymal stem cell-derived lncRNAs NKILA contributes to stemness and chemoresistance by fatty acid oxidation in gastric cancer via miR-485-5p/STAT3

Figure 1 Mesenchymal stromal cells enhances stemness and chemoresistance of gastric cancer cells. A-E: The MKN45 and AGS cells were co-cultured with mesenchymal stromal cells (MSCs); The stemness was analyzed using sphere formation assay (A); The mRNA expression of SOX2, Oct4, LIN28, and CD133 was measured by qPCR (B); The levels of SOX2, Oct4, LIN28, and CD133 was detected by Western blot analysis (C); The CD44+ and CD133+ cells were identified by flow cytometry analysis (D and E); F and G: The MKN45 and AGS cells were co-cultured with MSCs and treated with oxaliplatin or 5-Fu; The cell apoptosis was determined by flow cytometry analysis (F); The cell proliferation was examined by colony formation assay (G). ^aP < 0.01; MSC: Mesenchymal stromal cell.