Application of single-cell sequencing in the study of immune cell infiltration in inflammatory bowel disease and colorectal cancer

Figure 1 The workflow of single-cell RNA sequencing analysis for tumor samples. First, tumor samples are obtained and processed into single-cell (SC) suspensions. Subsequently, RNA is extracted, followed by SC RNA sequencing (scRNA-seq) and the construction of a complementary DNA (cDNA) library. Based on the data from the library, on one hand, immune cells in tumor samples can be identified and classified; on the other hand, specific signaling pathways or transcription factors can be determined, facilitating in-depth exploration of tumor-related biological mechanisms.