Circular RNA hsa_circRNA_101996 modulates gastric cancer cell proliferation and apoptosis through the miR-577/HMGN5 axis

Figure 4 Validation of the regulatory relationship among miR-577 and HMGN5. A: TargetScan was used to predict the binding site of miR-577 and HMGN5; B and C: Luciferase reporter gene assays confirmed the target relationship between miR-577 and HMGN5 in OCUM-1 and HSC-39 cell lines; D-I: The mRNA and protein levels of HMGN5. ^aP < 0.001; ^bP < 0.01. NC: Negative control.