Simvastatin inhibits proliferation and migration, promotes oxidative stress and ferroptosis in colon cancer

Figure 6 Knockdown of SERPINE1 induces oxidative stress and ferroptosis via the cyclic guanosine monophosphate-protein kinase G pathway in HCT-116 and SW620 cells. A: Reactive oxygen species content in cells; B: Malondialdehyde content in cells; C: Glutathione content in cells; D: Ferrous ion levels in cells; E: The expression of SLC7A11, ferroportin and TRF1 analyzed by Western blot. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ³P vs single-cell RNA sequencing reveals-negative control group. ⁴P vs single-cell RNA sequencing reveals SERPINE1 group. OD: Optical density; si-NC: Single-cell RNA sequencing reveals-negative control; si-SERPINE1: Single-cell RNA sequencing reveals SERPINE1; cGMP: Cyclic guanosine monophosphate-protein; FPN: Ferroportin; MDA: Malondialdehyde; GSH: Glutathione; Fe²⁺: Ferrous ion.