Simvastatin inhibits proliferation and migration, promotes oxidative stress and ferroptosis in colon cancer

Figure 5 Knockdown of SERPINE1 inhibits cell viability and proliferation, reduces migration, and promotes apoptosis via the cyclic guanosine monophosphate-protein kinase G pathway in HCT-116 and SW620 cells. A: Cell viability detected by cell counting kit-8 assay; B: Cell proliferation detected by colony formation assays; C: Cell migration measured by Transwell assay; D: Cell apoptosis detected by flow cytometry; E: The expression of Ki-67, proliferating cell nuclear antigen, MMP2, MMP9, Bcl-2 and Bax analyzed by Western blot. ^bP < 0.01. ^cP < 0.001. ³P vs single-cell RNA sequencing reveals-negative control group. ⁴P vs single-cell RNA sequencing reveals SERPINE1 group. OD: Optical density; si-NC: Single-cell RNA sequencing reveals-negative control; si-SERPINE1: Single-cell RNA sequencing reveals SERPINE1; cGMP: Cyclic guanosine monophosphate-protein; PCNA: Proliferating cell nuclear antigen.