Simvastatin inhibits proliferation and migration, promotes oxidative stress and ferroptosis in colon cancer

Figure 3 Simvastatin modulates SERPINE1 and activates the cyclic guanosine monophosphate-protein kinase G signaling pathway in HCT-116 and SW620 cells. A: Protein-protein interaction network analysis of simvastatin; B: Kyoto Encyclopedia of Genes and Genomes pathway enrichment of SERPINE1; C and D: The expression of SERPINE1 analyzed by real time polymerase chain reaction (PCR) and Western blot in NCM460, HCT-116 and SW620 cells; E and F: The expression of SERPINE1 analyzed by real time PCR and Western blot in simvastatin-treated HCT-116 and SW620 cells; G and H: The expression of GUCY1B1 and PRKG1 analyzed by real time PCR and Western blot in simvastatin-treated HCT-116 and SW620 cells. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ¹P vs control group. ²P vs NCM460 group. VEGF: Vascular endothelial growth factor; AGE-RAGE: Advanced glycation end products-receptor for advanced glycation end products; HIF-1: Hypoxia inducible factor-1; TNF: Tumor necrosis factor; cGMP-PKG: Cyclic guanosine monophosphate-protein kinase G.