Simvastatin inhibits proliferation and migration, promotes oxidative stress and ferroptosis in colon cancer

Figure 2 Simvastatin-induced oxidative stress and ferroptosis in HCT-116 and SW620 cells. A: Reactive oxygen species content in cells; B: Malondialdehyde content in cells; C: Glutathione content in cells; D: Ferrous ion levels in cells; E: The expression of SLC7A11, ferroportin and TRF1 analyzed by Western blot. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ¹P vs control group. OD: Optical density; FPN: Ferroportin; MDA: Malondialdehyde; GSH: Glutathione; Fe²⁺: Ferrous ion.