Long noncoding RNA SNHG5 promotes 5-fluorouracil resistance in colorectal cancer by regulating miR-26b/p-glycoprotein axis

Figure 7 SNHG5 contributes to 5-fluorouracil resistance in part by regulating the miR-26b/p-glycoprotein axis. A and B: The expression levels of miR-26b and p-glycoprotein (Pgp) were evaluated in the colorectal cancer tissues; C and D: Pearson’s correlation analysis was performed to analyze the correlation between SNHG5 and miR-26b, SNHG5 and Pgp; E: Western blotting was performed to assess the expression levels of Pgp in LOVO and HT-29 cells transfected with control + miR-control (miR-NC), SNHG5 + miR-NC, control + miR-26b, or SNHG5 + miR-26b. Densitometric analysis of the Western blot signals is shown; F: Western blotting was performed to assess the expression levels of Pgp, Bax and Bcl-2 in LOVO cells transfected with negative control-short hairpin RNA (shNC) + vector, SNHG5-short hairpin RNA (shSNHG5) + Vector, shNC + Pgp, or shSNHG5 + Pgp. Densitometric analysis of the Western blot signals is shown. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. miR-NC: MiR-control; Con: Control; shSNHG5: SNHG5-short hairpin RNA; shNC: Negative control-short hairpin RNA; 5-Fu: 5-fluorouracil; Pgp: P-glycoprotein.