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©The Author(s) 2025.
World J Gastrointest Oncol. May 15, 2025; 17(5): 102417
Published online May 15, 2025. doi: 10.4251/wjgo.v17.i5.102417
Published online May 15, 2025. doi: 10.4251/wjgo.v17.i5.102417
Figure 7 SNHG5 contributes to 5-fluorouracil resistance in part by regulating the miR-26b/p-glycoprotein axis.
A and B: The expression levels of miR-26b and p-glycoprotein (Pgp) were evaluated in the colorectal cancer tissues; C and D: Pearson’s correlation analysis was performed to analyze the correlation between SNHG5 and miR-26b, SNHG5 and Pgp; E: Western blotting was performed to assess the expression levels of Pgp in LOVO and HT-29 cells transfected with control + miR-control (miR-NC), SNHG5 + miR-NC, control + miR-26b, or SNHG5 + miR-26b. Densitometric analysis of the Western blot signals is shown; F: Western blotting was performed to assess the expression levels of Pgp, Bax and Bcl-2 in LOVO cells transfected with negative control-short hairpin RNA (shNC) + vector, SNHG5-short hairpin RNA (shSNHG5) + Vector, shNC + Pgp, or shSNHG5 + Pgp. Densitometric analysis of the Western blot signals is shown. aP < 0.05. bP < 0.01. cP < 0.001. miR-NC: MiR-control; Con: Control; shSNHG5: SNHG5-short hairpin RNA; shNC: Negative control-short hairpin RNA; 5-Fu: 5-fluorouracil; Pgp: P-glycoprotein.
- Citation: Wang B, Zhou Q, Cheng CE, Gu YJ, Jiang TW, Qiu JM, Wei GN, Feng YD, Ren LH, Shi RH. Long noncoding RNA SNHG5 promotes 5-fluorouracil resistance in colorectal cancer by regulating miR-26b/p-glycoprotein axis. World J Gastrointest Oncol 2025; 17(5): 102417
- URL: https://www.wjgnet.com/1948-5204/full/v17/i5/102417.htm
- DOI: https://dx.doi.org/10.4251/wjgo.v17.i5.102417