Long noncoding RNA SNHG5 promotes 5-fluorouracil resistance in colorectal cancer by regulating miR-26b/p-glycoprotein axis

Figure 3 SNHG5 knockdown suppresses 5-fluorouracil resistance in 5-fluorouracil-resistant colorectal cancer cells. A: The expression of SNHG5 was assessed in 5-fluorouracil (5-Fu)-resistant colorectal cancer (CRC) cells (LOVO/5-Fu, HT-29/5-Fu) which were transfected with SNHG5-short hairpin RNA and negative control lentivirus; B and C: The viability of LOVO/5-Fu and HT-29/5-Fu cells treated with 5-Fu at different concentrations was evaluated, and the IC₅₀ of 5-Fu-resistant CRC cells was calculated; D: The apoptosis rate of stable cells treated with 5-Fu was analyzed using annexin-V fluorescein 5-isothiocyanate/propidium iodide staining assays (left) and quantified (right); E: Western blot analysis was performed to assess the expression of markers of apoptosis (Bax and Bcl-2), with β-actin as an internal control (left), and densitometric analysis of Western blot signals is shown (right). ^bP < 0.01. ^cP < 0.001. shSNHG5: SNHG5-short hairpin RNA; shNC: Negative control-short hairpin RNA; 5-Fu: 5-fluorouracil.