Transglutaminase 2 serves as a pathogenic hub gene of KRAS mutant colon cancer based on integrated analysis

Figure 7 Molecular function of transglutaminase 2 in KRAS mutant colon cancer cell line HCT-116. A: Immunohistochemistry images of colon cancer tissue microarray showed the location of transglutaminase 2 (TGM2) and JAK3; B: The efficiency of small interfering RNA-induced TGM2 knockdown by western blotting; C: Cell proliferation curves in TGM2-downregulated HCT-116 cells through Cell Counting Kit-8 assays; D: Representative images and bar graph of colony formation were shown in TGM2-downregulated HCT-116 cells; E: Representative images and quantification of Transwell migration assay and Transwell Matrigel invasion assay were shown in TGM2-downregulated HCT-116 cells. Data are represented as means ± SD from three separate experiment. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. TGM2: Transglutaminase 2; siRNA: Small interfering RNA.