METTL5 promotes cell proliferation, invasion, and migration by up-regulating Toll-like receptor 8 expression in colorectal cancer

Figure 2 Toll-like receptor 8 is identified as a downstream target of METTL5 and an oncogenic protein in colorectal cancer. A: The identified differentially expressed genes were enriched using the clusterpro digestion package in R language; B: Correlation analysis results show a positive correlation between METTL5 and Toll-like receptor 8 (TLR8) mRNA levels; C-F: TLR8 mRNA and protein levels were detected by polymerase chain reaction, immunohistochemical staining, and western blot in different colorectal cancer cell lines; G: TLR8 mRNA levels in different colorectal cancer (CRC) cell lines compared with normal intestinal mucosal epithelial cell line; H: The relationship between overall survival and TLR8 expression was analyzed in consecutive cases of CRC; I-N: CCK-8 (I and J), transwell (K and L), and scratch experiments (M and N) demonstrated that down-regulation of TLR8 inhibited the proliferation, migration, and invasion of HT-29 and HCT-116 cells. ^aP < 0.05, ^bP < 0.01. TLR8: Toll-like receptor 8.