Circ_0003356 suppresses gastric cancer growth through targeting the miR-668-3p/SOCS3 axis

Figure 5 SOCS3 is targeted by miR-668-3p. A: Binding sites between SOCS3 and miR-668-3p were predicted by Starbase v2.0 software; B: Dual-luciferase reporter assays were used to confirm the targeting relationship between SOCS3 and miR-668-3p in AGS and HGC-27 cells; C: Relative expression of SOCS3 in AGS and HGC-27 cells detected by western blotting following miR-668-3p overexpression; D: Relative protein expression of SOCS3 in AGS and HGC-27 cells detected by western blotting after miR-668-3p inhibition; E: Relative mRNA expression of SOCS3 was detected by quantitative real-time polymerase chain reaction in tumor tissues and normal tissues; F: Correlation between circ_0003356 and SOCS3 in gastric cancer (GC) tissues analyzed by Pearson’s correlation analysis; G: Correlation between miR-668-3p and SOCS3 in GC tissues analyzed by Pearson’s correlation analysis. ^bP < 0.01, ^cP < 0.001.