F-box and leucine-rich repeat 6 promotes gastric cancer progression via the promotion of epithelial-mesenchymal transition

Figure 2 F-box and leucine-rich repeat 6 promotes gastric cancer cell proliferation. A and B: Reverse transcription-quantitative polymerase chain reaction and Western blot analysis of MKN45 and HGC-27 stably transfected with F-box and leucine-rich repeat 6 (FBXL6) knockdown and overexpression of FBXL6 in MGC-803 cells; C and D: CCK8 assay analyzed the proliferation of stable knockdown in MKN-45 and HGC-27 cell lines and overexpression in MGC-803 cell; E and F: Colony formation assays were used to detect cell growth of knockdown FBXL6 in MKN-45 and HGC-27 or overexpression FBXL6 in MGC-803 cells; G and H: 5-ethynyl-2’-deoxyuridine assays help in the analysis of the viability of knockdown of FBXL6 in MKN-45 and HGC-27 cell or overexpression of FBXL6 in MGC-803; ^bP < 0.01; ^cP < 0.001. FBXL6: F-box and leucine-rich repeat 6.