Glutamine deprivation impairs function of infiltrating CD8+ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis

Figure 8 Regulation of CD8⁺ T cell function by carbonyl cyanide-3-chlorophenylhydrazone. A-C: Activated CD8⁺T cells were cultured in RPMI 1640 supplemented with 5 μmol/L or 10 μmol/L carbonyl cyanide-3-chlorophenylhydrazone for 3 d, respectively. The levels of indicated molecules in CD8⁺ T cells were detected by flow cytometry. A: PD-1; B: PRF1; C: Granzyme B. The data represent the mean ± SE; ^dP < 0.0001. CCCP: Carbonyl cyanide-3-chlorophenylhydrazone.