Genome-wide CRISPR-Cas9 screening identifies that hypoxia-inducible factor-1a-induced CBX8 transcription promotes pancreatic cancer progression via IRS1/AKT axis

Figure 6 CBX8 promotes the growth and proliferation of pancreatic cancer cells through IRS1 in vivo. A: Viability of BXPC-3 and PANC1 cells after transfection with CBX8 knockdown and/or IRS1 plasmid; B: Colony formation assay was performed to evaluate the effect of CBX8 on PC cell proliferation; C: Images of tumors harvested from each group; D: Weight of tumors in each group; E: Tumor growth curve drawn based on the tumor size measured each week; F: Representative images of immunohistochemistry staining for Ki67 in xenograft tumors; G: Schematic model illustrating that HIF-1a-meditated CBX8 transcription promotes pancreatic cancer progression via IRS1/AKT axis. ^aP < 0.05, ^bP < 0.01.