Genome-wide CRISPR-Cas9 screening identifies that hypoxia-inducible factor-1a-induced CBX8 transcription promotes pancreatic cancer progression via IRS1/AKT axis

Figure 3 CBX8 is required for efficient proliferation of pancreatic cancer cells in vitro and in vivo. A: Protein expression of CBX8 in pancreatic cancer (PC) cells and normal pancreatic cells; B: Western blot analysis of CBX8 knockdown efficiency in PC cells; C: Viability of PANC1 cells after transfection with CBX8 knockdown and control plasmids; D: Viability of BXPC-3 cells after transfection with CBX8 knockdown and control plasmids; E: Colony formation assay for evaluation of the effect of CBX8 on PC cell proliferation; F: Images of tumors harvested from each group; G: Tumor growth curve drawn based on the tumor size measured each week; H: Weight of tumors in each group; I: Representative images of immunohistochemistry staining for Ki67 in xenograft tumors. ^bP < 0.01.