Direct interaction between Rab5a and Rab4a enhanced epidermal growth factor-stimulated proliferation of gastric cancer cells

Figure 5 Rab5a could directly interact with Rab4a to regulate its protein level in HGC-27 cells. Lysates of untransfected HGC-27 cells (A and E) and HGC-27 cells transiently transfected with Rab5a (B and F), Rab4a (C and G), or Rab5a + Rab4a (D and H) were incubated with 20-40 μL of washed protein G agarose beads. Then the mixture was added with either anti-Rab4a antibody (upper row) or anti-Rab5a antibody (lower row). The immunocomplexes were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis and were immunoblotted with either anti-Rab5a antibody (upper row) or anti-Rab4a antibody (lower row) as indicated. Results presented are representatives of at least three independent experiments.