Direct interaction between Rab5a and Rab4a enhanced epidermal growth factor-stimulated proliferation of gastric cancer cells

Figure 4 Confocal immunofluorescent analysis of the co-localization between Rab5a and Rab4a in endosomes of HGC-27 cells. A: Green fluorescent protein (GFP)–Rab5a and red fluorescent protein (RFP)–Rab4a overexpressed in HGC-27 cells were visualized using immunofluorescent confocal microscopy. Four groups of HGC-27 cells are shown, namely the Control group (a1-a4), the Rab5a overexpression group (b1-b4), the Rab4a overexpression group (c1-c4), and the Rab5a + Rab4a overexpression group (d1-d4). Scale bar = 20 μm; B: The colocalization between Rab5a and Rab4a was evaluated by EzColocalization plugin of ImageJ. Scatterplot of GFP–Rab5a and RFP–Rab4a is shown for cells in the Rab5a + Rab4a overexpression group (d4).