Characterization and strong risk association of TLR2 del -196 to -174 polymorphism and Helicobacter pylori and their influence on mRNA expression in gastric cancer

Table 2 Nucleotide primer sequences, PCR conditions, and minor allele frequency for polymorphisms TLR2 -196 to -174 ins/del (rs111200466) and TLR2 19216T/C (rs3804099)

Genes	Location	MAF	Primers, 5’-3’	Cycles	T° melting	Enzyme	Genotypes, bp
TLR2 -196 to -174 ins/del (rs111200466)	Chromosome 4:153684312-153684338 (intron variant)	0.1952 (del)	F: CACGGAGGCAGCGAGAAA	35	60 °C	-	ins/ins: 286 bp
			R: CTGGGCCGTGCAAAGAAG				ins/del: 286 + 264 bp
							del/del: 264 bp
TLR2 19216T/C (rs3804099)	Chromosome 4:153703504 (synonymous variant)	0.4048(C)	F: TCCCTGGGCAGTCTTGAACATTTAG	30	65 °C	TaiI	TT: 415 bp
							TC: 415 + 109 + 306 bp
			R: TGTCCAAATCAGTATCTCGCAGTTCC				CC: 306 + 109 bp

MAF: Minor allele frequency - extracted from 1000 Genomes Project Phase 3; bp: Base pairs R: Reverse; F: Forward.