Retrospective Cohort Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Feb 15, 2016; 8(2): 215-221
Published online Feb 15, 2016. doi: 10.4251/wjgo.v8.i2.215
Correlation of Helicobacter pylori and interleukin-8 mRNA expression in high risk gastric cancer population prediction
Wilaiwan Chongruksut, Sirikan Limpakan (Yamada), Bandhuphat Chakrabandhu, Chidchanok Ruengorn, Sirisak Nanta
Wilaiwan Chongruksut, Sirikan Limpakan (Yamada), Bandhuphat Chakrabandhu, Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
Chidchanok Ruengorn, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
Sirisak Nanta, Mae Sai District Hospital, Mae Sai, Chiang Rai 50200, Thailand
Author contributions: All the authors contributed to the article.
Conflict-of-interest statement: There is no conflict-of-interest.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Sirikan Limpakan (Yamada), MD, PhD, FARCS(T), Associate Professor, Division Chief of Gastrointestinal Surgery and Endoscopy, Department of Surgery, Faculty of Medicine, Chiang Mai University, 239 Huai Kaeo Road, T. Suthep, A. Mueang, Chiang Mai 50200, Thailand. siyamada@yahoo.com
Telephone: +66-81-6716737
Received: July 30, 2015
Peer-review started: July 30, 2015
First decision: September 14, 2015
Revised: October 15, 2015
Accepted: December 3, 2015
Article in press: December 4, 2015
Published online: February 15, 2016
Abstract

AIM: To evaluate (1) the association of the Helicobacter pylori (H. pylori) test and interleukin-8 (IL-8) mRNA expression alone and the severity of gastric cancer (GC); (2) the association of both tests were added to patients' characteristics to identifli Thai suspected patients of gastric cancer who would receive the most benefit; and (3) diagnostic value of levels of IL-8 mRNA expression for gastric cancer.

METHODS: A cross-sectional analytical study was completed with 220 patients with 86 GC patients who underwent endoscopy with gastric surgery divided into non-metastasis and metastasis groups, and 134 patients with benign lesions who underwent endoscopic examination, at the Gastrointestinal Surgery and Endoscopy Unit, Chiang Mai University Hospital between 2006 and 2010. Of 220 patients, 86 cases of diagnosed gastric adenocarcinoma were in an advanced stage and 134 cases were non-cancer patients.

RESULTS: The IL-8 mRNA expression showed predominant association with advanced GC when compared to H. pylori infection alone [OR (95%CI); 0.86 (0.49-1.53) vs 5.44 (3.08-9.62)] when including the patients’ characteristics the highest of the area under the receiver operating characteristic curves (AuROC) of the model were males older than 40 years of age [AuROC (95%CI); 0.81 (0.75-0.86)]. However, preliminary testing for diagnostic indices of four cut-off points of IL-8 mRNA expression to predict the severity of GC cases found an increasing suboptimal trend from the likelihood ratio of positive to differentiate the severity in the GC group. The IL-8 mRNA expression showed a predominant association with GC when compared to H. pylori infection, especially in males older than 40 years of age who may benefit most from this test.

CONCLUSION: The future research of IL-8 mRNA expression to predict severity in the gastric cancer group should be warranted.

Keywords: Helicobacter pylori, Interleukin-8 mRNA expression, Gastric cancer

Core tip: The author reviewed updated basic research studies regarding linkages of inflammatory cytokine genetic expression level and gastric cancer (GC) risk prediction in the Thai population. The review focused on interleukin-8 (IL-8) mRNA expression and Helicobacter pylori (H. pylori) infection in which are found an increasing risk for GC and aggressive histologic types. We performed the epidemiologic data in-depth analysis, and make the various cut off points to discern which level of IL-8 mRNA expression has remarkable predictive risk value in comparison with H. pylori infection to predict GC occurrence.