Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2

doi:10.4254/wjh.v9.i7.368

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Hepatol. Mar 8, 2017; 9(7): 368-384
Published online Mar 8, 2017. doi: 10.4254/wjh.v9.i7.368

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Figure 1 Liver heparan sulfate proteoglycan was detected by anti-rat glypican and mAb 1E4-1D9. A: Liver heparan sulfate proteoglycan (HSPG) was digested with heparin lyase I, II, III and probed with anti-rat glypican[47]; B: Silver stain of liver HSPG; C: Liver HSPG was reacted with mAb 1E4-1D9 and visualized by horseradish peroxidase-conjugated rabbit anti-mouse Igs following with 3,3-diamino benzidine/H₂O₂ substrate.

Citation: Vongchan P, Linhardt RJ. Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2. World J Hepatol 2017; 9(7): 368-384
URL: https://www.wjgnet.com/1948-5182/full/v9/i7/368.htm
DOI: https://dx.doi.org/10.4254/wjh.v9.i7.368