Discussion on the effect of Honghua Qinggan Shisanwei Wan on regulating macrophage polarization and alleviating liver injury

Figure 7 Honghua Qinggan Shisanwei Wan affects M1 and M2 macrophage polarization and expression of its associated factors. A: Cell counting kit-8 was used to detect the effects of different concentrations of Honghua Qinggan Shisanwei Wan (HHQG) on the viability of RAW264.7 cells; B: MRNA expression of inducible nitric oxide synthase, tumor necrosis factor-α, C-C motif chemokine ligand 2, and interleukin-6 in the M1 macrophage polarization model; C: MRNA expression of C-X3-C motif chemokine receptor 1, YM-1, transforming growth factor-β, interleukin-10 in the M1 macrophage polarization model; D: The inhibitory effect of HHQG on the expression of inducible nitric oxide synthase and pro-inflammatory factors in M1-type macrophage markers; E: The promoting effect of HHQG on the expression of M2 macrophage marker YM-1 and anti-inflammatory factors. ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001, NS: Not significant. OD: Optical density; iNOS: Inducible nitric oxide synthase; Con: Control; HHQG: Honghua Qinggan Shisanwei Wan; TNF-α: Tumor necrosis factor-α; CCL2: C-C motif chemokine ligand 2; IL: Interleukin; CX3CR1: C-X3-C motif chemokine receptor 1; TGF: Transforming growth factor.