Discussion on the effect of Honghua Qinggan Shisanwei Wan on regulating macrophage polarization and alleviating liver injury

Figure 2 Protective effect of Honghua Qinggan Shisanwei Wan against carbon tetrachloride-induced liver injury in mice. A: Mouse body weight alterations following acute liver injury induced by carbon tetrachloride; B: Alanine aminotransferase assay in mouse serum; C: Aspartate aminotransferase assay in mouse serum; D: The liver histopathology was analyzed by hematoxylin and eosin staining (200 ×); E: The liver histopathology was analyzed by Masson staining (200 ×); F: The tumor necrosis factor-α/C-C motif chemokine ligand 2/interleukin-1β/interleukin-6 expression of liver tissue in the liver injury; G: The expression of tumor necrosis factor-α in liver injury (200 ×). The regions indicated by arrows represent the injured areas of liver tissue and the corresponding positive expression regions of the gene. ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001, NS: Not significant. ALT: Alanine aminotransferase; CCL2: C-C motif chemokine ligand 2; CCL₄: Carbon tetrachloride; HHQG: Honghua Qinggan Shisanwei Wan; AST: Aspartate aminotransferase; TNF-α: Tumor necrosis factor-α; IL: Interleukin; Con: Control.