Bioengineered functional humanized livers: An emerging supportive modality to bridge the gap of organ transplantation for management of end-stage liver diseases

Table 1 Method adopted for whole complex organ acellularization techniques for different organisms

Organ	Acellularization agent	Perfusion method	Animal model	Reference
Heart	SDS, PEG, Triton X-100, and enzyme-based protocols deoxycholic acid	Antegrade coronary perfusion	Rat	[98]
	Trypsin, EDTA, NaN3, Triton X-100, and deoxycholic acid	Retrograde aortic perfusion	Pig	[65]
Lung	0.1% and 0.5% SDS	Antegrade pulmonary arterial perfusion	Rat	[63]
	CHAPS	Pulmonary artery and tracheal perfusion	Rat	[66]
	Triton X-100 and sodium deoxycholate	Right ventricle and tracheal perfusion	Mouse	[99]
Liver	Triton X-100 plus 0.1% SDS	Portal vein perfusion	Rat	[100]
	SDS		Rat	[70]
	1% Triton X-100 and 0.1% ammonium hydroxide		Mouse, rat, ferret, rabbit and pig	[69]
	0.25% and 0.5% SDS		Pig	[101]
	Sodium citrate + SDS + Triton-X-100	Hepatic artery perfusion	Rat	[74]
Kidney	0.5, 3, 6, 10% Triton X-100, 5 mM	Renal artery perfusion	Rat	[71]
	calcium chloride, 5 mM magnesium sulfate, 1 M sodium chloride, DNase, and 4% sodium deoxycholate		Rat	[72]
	3% Triton X-100, DNase, and 4% SDS
	1% SDS and 1% Triton X-100
	1% Triton X-100 and 0.1% ammonium hydroxide		Pig	[68]
	Heparin and antibiotic-containing physiological saline, 0.1-1.0% SDS, 0.1% Triton-X-100 and 0.0025% deoxyribonuclease 1		Goat	[75]