Autologous tissue patch rich in stem cells created in the subcutaneous tissue

Figure 8 Localization of mesenchymal stem cells in the de novo tissue patch induced in the subcutaneous space by an inert body. CD271 cells were immune-stained by a red fluorescent probe, CD34 cells by a green fluorescent probe and all nuclei (counterstained in the merge picture only) by DAPI. MSCs identified as CD271+CD34+ were clearly identified in the medial layer of the tissue patch at day 4 after the implantation of the inert body (white arrows in the merged picture), but rarely seen at day 7 or after. Light blue arrows indicate the inner aspect of the tissue patch. MSCs: Mesenchymal stem cells; DAPI: 4',6-diamidino-2-phenylindole.