Fetal cardiac mesenchymal stem cells express embryonal markers and exhibit differentiation into cells of all three germ layers

Figure 4 Representative immunocytochemistry photomicrographs (A) and representative reverse-transcription polymerase chain reaction gel photomicrographs (B). A: Representative immunocytochemistry photomicrographs (40 ×, 20 μm) showing differentiation of rat fetal cardiac mesenchymal stem cells into neuronal cells [A1: Neuronal filament (NF)-160 and Hoechst and A2: no NF-160 only Hoechst dye in control cells]; [A3: Glial fibrillar acidic protein (GFAP) and Hoechst dye and A4: no GFAP only Hoechst dye in control cells]; B: Representative reverse-transcription polymerase chain reaction gel photomicrographs showing expression of NF-160 and GFAP by neuronal cells differentiated from rat fetal cardiac mesenchymal stem cells. Control cells without induction medium did not show expression of above markers.