Experimental study on ultrasound-targeted microbubble destruction promoting the proliferation, migration of human umbilical cord mesenchymal stem cells

Figure 5 Effects of ultrasound-targeted microbubble destruction exposure duration on the proliferation and migration of human umbilical cord mesenchymal stem cells. A: Proliferation rates of human umbilical cord mesenchymal stem cells (hUCMSCs) following ultrasound-targeted microbubble destruction (UTMD) treatment for different durations. ^aP < 0.001 vs the M (control) group; ^bP < 0.001 vs the M group; ^cP < 0.001 vs the M group; B and C: Evaluation of cell migration capacity using a wound healing (scratch) assay. Representative microscopic images of the scratch wound in hUCMSC monolayers at 0 hour and 24 hours after treatment with microbubbles alone, ultrasound alone (60 seconds), or UTMD for varying durations (60-600 seconds). ^aP < 0.05 vs M, ^bP < 0.05 vs M, ^cP < 0.05 vs M, ^dP < 0.01 vs M, ^eP < 0.05 vs M, ^fP < 0.05 vs M. Scale bar = 100 μm. Quantitative analysis of the cell migration rate at 24 hours post-treatment. All data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons.