Reduced NRF2/Mfn2 activity promotes endoplasmic reticulum stress and senescence in adipose-derived mesenchymal stem cells in hypertrophic obese mice

Figure 5 Mitofusin-2 is physically bound to binding immunoglobulin protein. A: Eukaryotic Orthologous Group; B: Gene Ontology; C: Kyoto Encyclopedia of Genes and Genome analysis of mitofusin-2 (MFN2) binding protein; D: Western blot verified MFN2 binding immunoglobulin (IgG) protein (BIP) in a coimmunoprecipitation assay; E: Co-localized immunofluorescence staining of MFN2 and BIP, bar = 25 μm; F: Long-axis MFN2 and BIP intensity of adipose-derived mesenchymal stem cells (ASCs) analyzed by ImageJ software; G: SA-B-Gal staining of short hairpin RNA against negative control (shNC) ASCs, Mfn2 knockdown ASCs, and Mfn2 knockdown ASCs treated with BIP inducer X, bar = 100 μm; H: Quantitative polymerase chain reaction analysis of the relative mRNA expression relative to β-actin in shNC ASCs, Mfn2 knockdown ASCs, and Mfn2 knockdown ASCs treated with BIP inducer X. ^aP < 0.05; ^bP < 0.01.