Reduced NRF2/Mfn2 activity promotes endoplasmic reticulum stress and senescence in adipose-derived mesenchymal stem cells in hypertrophic obese mice

Figure 4 Nuclear factor erythroid-derived 2 regulates senescence phenotype and endoplasmic reticulum stress through mitofusin-2. A: SA-B-Gal staining and Oil Red O staining of control and mitofusin-2 (Mfn2) knockdown adipose-derived mesenchymal stem cells (ASCs), bar = 100 μm; B: Percentage of SA-B-Gal staining-positive cells analyzed by ImageJ software (n = 3); C: Percentage of positive Oil Red O stained area to cell number (n = 3); D: Quantitative polymerase chain reaction analysis of the relative mRNA expression relative to β-actin (ACTB) in control and Mfn2 knockdown ASCs; E: Western blot of control and Mfn2 knockdown ASCs; F: Relative quantitative analysis of MFN2, inositol-requiring enzyme-1 alpha (IRE1a), and p16 relative to β-actin for Western blotting by ImageJ software (n = 3); G: Western blot of nuclear factor erythroid-derived 2 (Nrf2) knockdown ASCs and Nrf2 knockdown with superimposed Mfn2 overexpression ASCs; H: Relative quantitative analysis of proteins relative to ACTB for Western blotting by ImageJ software in Nrf2 knockdown ASCs and Nrf2 knockdown with superimposed Mfn2 overexpression ASCs (n = 3). ^aP < 0.05; ^bP < 0.01.