Reduced NRF2/Mfn2 activity promotes endoplasmic reticulum stress and senescence in adipose-derived mesenchymal stem cells in hypertrophic obese mice

Figure 3 Nuclear factor erythroid-derived 2 regulates mitofusin-2 transcriptional activity. A and B: Mitochondrial staining of adipose-derived mesenchymal stem cells (ASCs) with MitoTracker Red dye in control and hypertrophic obesity group, bar = 25 μm; C and D: Long-axis MitoTracker Red intensity of ASCs in control and hypertrophic obesity groups analyzed by ImageJ; E: Quantitative polymerase chain reaction (qPCR) analysis of the relative mRNA expression of mitofusin-2 (Mfn2) relative to β-actin (ACTB) in control and hypertrophic obesity ASCs (n = 3); F: qPCR analysis of the relative mRNA expression of Mfn2 relative to ACTB in control and nuclear factor erythroid-derived 2 (Nrf2) knockdown ASCs (n = 3); G: Western blot of MFN2 in control and Nrf2 knockdown ASCs; H: Relative quantitative analysis of MFN2 relative to β-actin for Western blot by ImageJ software (n = 3); I: Relative enrichment of NRF2 on the Mfn2 promoter region was detected by chromatin immunoprecipitation-qPCR (n = 3). ^aP < 0.05; ^bP < 0.01. IgG: Immunoglobulin G; IRE1α: Inositol-requiring enzyme-1 alpha.