Cell-free extracts from human fat tissue attenuate ischemic injury in cardiomyocytes in a murine model

Figure 6 Activation of the basic fibroblast growth factor-phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin signaling pathways may be involved in the role of fat extract in myocardial ischemia injury. The expression and phosphorylation levels of protein kinase B and mechanistic target of rapamycin were analyzed by western blot. A: C57 mice myocardial tissues; B and C: Hypoxia treated cardiomyocytes. Data were presented as mean ± SEM (n = 3 per group), ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, NS: No significance. MI: Myocardial infarction; PBS: Phosphate-buffered saline; FE: Fat extract; Akt: Protein kinase B; mTOR: Mechanistic target of rapamycin.