Cell-free extracts from human fat tissue attenuate ischemic injury in cardiomyocytes in a murine model

Figure 4 Fibroblast growth factor receptor family inhibitor partly blocked the therapeutic effect of fat extract at the cellular level after ischaemia injury. A: Relative mRNA expression levels of tropomyosin receptor kinase B, transforming growth factor-beta receptor, Met, fibroblast growth factor receptor 1, vascular endothelial growth factor receptor 2, GFR1 in cardiomyocytes; B Quantification of beat rate of cardiomyocytes; C-E: The level of cell apoptosis were calculated using flow cytometry (n = 3); F: Quantification of cell apoptosis. Values indicate mean ± SEM from at least three independent experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. TRKB: Tropomyosin receptor kinase B; TGFBR: Transforming growth factor-beta receptor; FGFR1: Fibroblast growth factor receptor 1; VEGFR2: Vascular endothelial growth factor receptor 2; FE: Fat extract; Infi: Infigratinib.