Optimal good manufacturing practice-compliant production of dental follicle stem cell sheet and its application in Sprague-Dawley rat periodontitis

Figure 4 The effect of L-ascorbic acid concentration on the characteristics of cell sheet. A: Acridine orange/propidium iodide staining and quantitative analysis of dead cells in the cell sheets at various L-ascorbic acid concentrations. Scale bar = 250 μm; B: Quantitative reverse-transcription polymerase chain reaction analysis of osteogenic and senescence-related genes in cell sheets cultured with various L-ascorbic acid concentrations; C: Final cell counts in the cell sheets at various L-ascorbic acid concentrations; D: Measurement of hydroxyproline content in the cell sheets at different L-ascorbic acid concentrations; E: Measurement of the sulfated glycosaminoglycan content in cell sheets with varying L-ascorbic acid concentrations. Data are presented as mean ± SD, n = 3. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. ALP: Alkaline phosphatase; RUNX-2: Runt-related transcription factor 2; CAP: Cartilage associated protein; HYP: Hydroxyproline; sGAG: Sulfated glycosaminoglycan.