Optimal good manufacturing practice-compliant production of dental follicle stem cell sheet and its application in Sprague-Dawley rat periodontitis

Figure 2 The effect of time on the characteristics of dental follicle stem cell sheet. A: Variation in the cell count within the cell sheet over time; B: Flow cytometry analysis of cell cycle phases in cell sheets at various time points; C: Quantitative reverse-transcription polymerase chain reaction analysis of the genes related to osteogenesis, chondrogenesis, odontogenesis, and aging at different time points; D: Quantitative reverse-transcription polymerase chain reaction analysis of telomere length in the cell sheets at different time points; E: Acridine orange/propidium iodide staining of cell sheets at different time points by the quantitative analysis of dead cells. Scale bar = 250 μm; F: Quantitative assessment of β-galactosidase staining in cell sheets at different time points; G: Morphology of the dental follicle stem cell sheets at various time points. Data are presented as mean ± SD, n = 3. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. ALP: Alkaline phosphatase; RUNX-2: Runt-related transcription factor 2; CAP: Cartilage associated protein; COL-1: Type I collagen; OPN: Osteopontin; OCN: Osteocalcin.