Optimal good manufacturing practice-compliant production of dental follicle stem cell sheet and its application in Sprague-Dawley rat periodontitis

Figure 1 The effect of cell generation on the characteristics of dental follicle stem cell sheet. A: Schematic representation of dental follicle stem cell sheets cultured from cells at different passages. Created with BioRender.com; B: Quantitative analysis of dead cells in sheets cultured from cells at different passages. Scale bar = 250 μm; C: Alizarin red staining of cell sheets cultured at different passages. Scale bar = 100 μm. Data are presented as mean ± SD, n = 3. ^aP < 0.05.