Basic Study
Copyright ©The Author(s) 2021.
World J Stem Cells. Oct 26, 2021; 13(10): 1580-1594
Published online Oct 26, 2021. doi: 10.4252/wjsc.v13.i10.1580
Table 2 qRT-PCR program layout
Process
Temperature (°C)
Time duration
Cycles
Initial denaturation9510 min1
Cyclic denaturation9515 s40
Annealing and extension601 min
Melting curve9515 s-
6015 s
9515 s