Therapeutic and Diagnostic Guidelines
Copyright ©The Author(s) 2020.
World J Stem Cells. Aug 26, 2020; 12(8): 706-720
Published online Aug 26, 2020. doi: 10.4252/wjsc.v12.i8.706
Figure 2
Figure 2 Processing of single-cell droplets. A: A single-cell in an oil droplet travels to a trapping module of the 10-nl reactor (green, in inset). Other cells are forced to bypass to the next unit; B: FEOET push the droplet (with a cell) past the open valve (orange bar), which closes, locking the droplet into the 10-nl ring with RT/RT-PCR master mix (green); and C: The ring’s peristaltic pump breaks the droplet to mix the cell with master-mix. When the reaction is finished, oil (blue) pushes the product (cDNA) out of the ring in the form of a droplet (10-nL) for downstream molecular analysis (Refer to[17] for details).