Basic Study
Copyright ©The Author(s) 2018.
World J Stem Cells. Dec 26, 2018; 10(12): 196-211
Published online Dec 26, 2018. doi: 10.4252/wjsc.v10.i12.196
Figure 5
Figure 5 Steady-state pHi in HEPES and 5% CO2/HCO3--buffered solution and the change in the expression of pHi regulators during the loss of pluripotency in human induced pluripotent stem cells. A: The resting pHi was a steady-state taken from the completely recovered pHi after intracellular acidification or alkalization. The dotted line indicates the value of the resting pHi; B: The max/min chart of the resting pHi in hiPSCs was collected from A (n = 20) and Figures 4C and D (n = 5). The means of the resting pHi in HEPES and 5% CO2/HCO3--buffered solution were found to be 7.50 ± 0.01 and 7.68 ± 0.01, respectively. After treatment with H30 and S90 in 5% CO2/HCO3--buffered solution, the resting pHi shifted to 7.66 ± 0.02 and 7.46 ± 0.02, respectively; C: Immunoblot analysis of the expression of NHE1, NHE3, V-ATPase, NBCe1, NBCe2, NBCn1 and OCT4 in hiPSCs in different culture media for different days (E8 and E6-1d to E6-4d). The histograms in B display the mean and the min to max values. hiPSCs: Human induced pluripotent stem cells; NHE: The Na+/H+ exchanger; NBC: The Na+/HCO3- cotransporter; V-ATPase: Vacuolar-ATPase.