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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Jan 26, 2015; 7(1): 223-234
Published online Jan 26, 2015. doi: 10.4252/wjsc.v7.i1.223
Published online Jan 26, 2015. doi: 10.4252/wjsc.v7.i1.223
Fetal vs adult mesenchymal stem cells achieve greater gene expression, but less osteoinduction
Juan E Santiago-Torres, Rebecca Lovasz, Alicia L Bertone, Comparative Orthopedic Research Laboratory, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, United States
Juan E Santiago-Torres, Alicia L Bertone, College of Medicine, The Ohio State University, Columbus, OH 43210, United States
Author contributions: Santiago-Torres JE and Bertone AL designed the research; Santiago-Torres JE and Lovasz R performed the research; Santiago-Torres JE and Bertone AL analyzed the data and wrote the paper.
Supported by The Ohio State University College of Medicine Roessler Research Scholarship (In part); in part by National Cancer Institution of the United States grant No. P30 CA016058 (Our histological examination was performed at The OSU Comparative Pathology and Mouse Phenotyping Shared Resource)
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Alicia L Bertone, DVM, PhD, Professor, Comparative Orthopedic Research Laboratory, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 601 Tharp St, Columbus, OH 43210, United States. bertone.1@osu.edu
Telephone: +1-614-2927449 Fax: +1-614-6885642
Received: July 31, 2014
Peer-review started: July 31, 2014
First decision: September 28, 2014
Revised: October 14, 2014
Accepted: October 31, 2014
Article in press: December 16, 2014
Published online: January 26, 2015
Peer-review started: July 31, 2014
First decision: September 28, 2014
Revised: October 14, 2014
Accepted: October 31, 2014
Article in press: December 16, 2014
Published online: January 26, 2015
Core Tip
Core tip: Fetal mesenchymal stem cells (MSCs) can be genetically manipulated ex vivo with adenoviral vectors without major effects on stemness. Their greater expression of exogenous genes than adult MSCs is promising for MSC-mediated gene therapy. MSC-mediated bone morphogenic protein 2 gene delivery provides osteogenic induction in vivo, which could have clinical applications in bone regeneration. Nonetheless, the abbreviated expression of the exogenous gene provided via adenoviral transduction may limit their applications in vivo.