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©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Stem Cells. Oct 26, 2019; 11(10): 859-890
Published online Oct 26, 2019. doi: 10.4252/wjsc.v11.i10.859
Published online Oct 26, 2019. doi: 10.4252/wjsc.v11.i10.859
Characterization of inflammatory factor-induced changes in mesenchymal stem cell exosomes and sequencing analysis of exosomal microRNAs
Chen Huang, Wen-Feng Luo, Yu-Feng Ye, Qi-De Song, Xue-Ping He, Han-Wei Chen, Yu-Kuan Tang, Department of Minimally Invasive Interventional Radiology, Guangzhou Panyu Central Hospital, Guangzhou 511400, Guangdong Province, China
Chen Huang, Han-Wei Chen, Jinan University, Guangzhou 510632, Guangdong Province, China
Chen Huang, Wen-Feng Luo, Yu-Feng Ye, Han-Wei Chen, Medical Imaging Institute of Panyu, Guangzhou 511400, Guangdong Province, China
Li Lin, Jinan University Biomedical Translational Research Institute, Jinan University, Guangzhou 510632, Guangdong Province, China
Zhe Wang, Yi Kong, Department of Pharmacy, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, Guangdong Province, China
Ming-Hua Luo, Department of Radiology, Shiyan People’s Hospital, Shenzhen 518108, Guangdong Province, China
Author contributions: Huang C designed the research and wrote the manuscript; Huang C, Luo WF, and Lin L performed all the experiments; Huang C, Wang Z, Kong Y, Chen HW, and Tang YK performed the analysis; Ye YF, He XP, Luo MH, and Song QD performed data collection and pre-processing; all authors approved the manuscript.
Supported by Panyu Science and Technology Plan Medical General Project , No. 2018-Z04-47 ; and Guangzhou Health Science and Technology Project , No. 20191A011120 .
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Yu-Kuan Tang, MD, Doctor, Department of Minimally Invasive Interventional Radiology, Guangzhou Panyu Central Hospital, 8 East Fuyu Road, Qiaonan Street, Panyu District, Guangzhou 511400, Guangdong Province, China. tyk20126@126.com
Telephone: +86-20-34858815 Fax: +86-20-84824443
Received: January 29, 2019
Peer-review started: January 29, 2019
First decision: March 14, 2019
Revised: March 24, 2019
Accepted: July 30, 2019
Article in press: July 30, 2019
Published online: October 26, 2019
Peer-review started: January 29, 2019
First decision: March 14, 2019
Revised: March 24, 2019
Accepted: July 30, 2019
Article in press: July 30, 2019
Published online: October 26, 2019
Core Tip
Core tip: The morphology and quantity of mesenchymal stem cell exosomes (MSCs-exo) are impacted in different inflammatory cytokine environments. Inflammatory cytokines impair the ability of MSCs-exo to promote angiogenesis. For instance, the tumor necrosis factor α and interleukin 6 groups exhibited decreased numbers of angiogenesis-related microRNAs (miRNAs), such as miR-196a-5p, miR-17-5p, miR-146b-5p, miR-21-3p, and miR-320. The same groups also had downregulated angiogenesis-related signaling pathways, such as PI3K-AKT and VEGF. Inflammatory cytokines may lead to changes in exosomal miRNAs that abnormally impact cellular components, molecular function, and biological processes, particularly angiogenesis-related miRNAs.