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©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
Human mesenchymal stem cells exhibit altered mitochondrial dynamics and poor survival in high glucose microenvironment
Ejlal Abu-El-Rub, Fatimah Almahasneh, Ramada R Khasawneh, Ayman Alzu'bi, Doaa Ghorab, Rawan Almazari, Huthaifa Magableh, Ahmad Sanajleh, Haitham Shlool, Mohammad Mazari, Noor S Bader, Joud Al-Momani
Ejlal Abu-El-Rub, Fatimah Almahasneh, Ramada R Khasawneh, Ayman Alzu'bi, Rawan Almazari, Huthaifa Magableh, Ahmad Sanajleh, Haitham Shlool, Mohammad Mazari, Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid 21163, Jordan
Doaa Ghorab, Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
Noor S Bader, Joud Al-Momani, Department of Basic Medical Sciences, Yarmouk University, Irbid 21163, Jordan
Author contributions: Abu-El-Rub E conceptualized the study; Abu-El-Rub E, Khasawneh RR, Almazari R, Sanajleh A, Magableh H, Shlool H, Mazari M, Bader NS, and Al-Momani J carried out the experiments; Abu-El-Rub E and Almahasneh F analyzed the data; Abu-El-Rub E, Almahasneh F, Alzu'bi A, and Ghorab D drafted and wrote the manuscript; Abu-El-Rub E and Almahasneh F revised and formatted the content of the manuscript and verified spelling, punctuation and grammatical errors; and all authors have read and approve the final manuscript.
Institutional review board statement: The ethical approval was not needed in this study. The human cell lines used for this study were commercially purchased.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
https://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Ejlal Abu-El-Rub, PhD, Assistant Professor, Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid-Shafiq Irshidat Street, Irbid 21163, Jordan. ejlal.abuelrub@yu.edu.jo
Received: September 28, 2023
Peer-review started: September 28, 2023
First decision: October 23, 2023
Revised: November 11, 2023
Accepted: November 24, 2023
Article in press: November 24, 2023
Published online: December 26, 2023
ARTICLE HIGHLIGHTS
Research background
Mesenchymal stem cells (MSCs) have the ability to cure many chronic diseases, including diabetes mellitus (DM) and its related multisystem complications.
Research motivation
The therapeutic outcomes of MSCs transplantation in DM are short-lived which require thorough investigation.
Research objectives
This study aimed to determine the effects of hyperglycemia microenvironment on various mitochondrial-related parameters in MSCs to better understand their fate in DM patients.
Research methods
Adipose tissue-derived MSCs were exposed to low and high glucose media and mitochondrial dynamics and regulators were measured and analyzed.
Research results
High glucose induces the apoptosis of adipose tissue-derived MSCs by disturbing many mitochondrial parameters, including mitochondrial membrane potential, nicotinamide adenine dinucleotide (NAD+)/NADH pool, mammalian target of rapamycin, and mitochondrial complexes I, IV, and V.
Research conclusions
Hyperglycemia decreases the survival of MSCs by triggering mitochondrial dysfunction in these cells causing their short-lived therapeutic outcomes.
Research perspectives
New strategies to improve the survival rate of MSCs in hyperglycemia should be the focus of future studies which can help in increasing the chances of using these cells clinically for the treatment of DM.
